Analytical Method Development and Validation and Forced Degradation Stability-Indicating Studies of Favipiravir by RP-HPLC and UV in Bulk and Pharmaceutical Dosage Form

Aims: To develop and validate a new, simple, rapid, precise, accurate An Eco-friendly RP-HPLC UV-Method Development Validation for an estimation of Favipiravir in Bulk pharmaceutical dosage form followed by Forced Degradation Studies.
 Study Design: This was employed UV-visible (200-400 nm 400-800 respectively) method development using C 18 inertsil column optimization variables bulk formulations.
 Place Duration the Study: The present work carried out at Ali-allana College Pharmacy, Akkalkuwa between duration November-2020 to February-2021.
 Methodology: UV-Spectroscopic developed form. solvent selected UV analysis water, solution range 2-10µg/ml scanned region from 200-400 λmax value determined. on ODS-3V C18 150 mm x 4.6mm 5μ buffer pH 3.5: acetonitrile [90:10] as mobile phase flow rate 1.0 ml/min PDA detection 358 nm.
 Results: maximum absorbance observed nm. wavelength further Favipiravir. calibration curve determined drug concentrations ranging 2-10 µg/ml. % recovery accuracy 100.50-100.76%. be precise with RSD 0.51-1.37 0.77-1.78 intraday Interday respectively. limit (LOD) quantification (LOQ) found 0.0723 &0.219 µg/ml respectively method. shown linear 50-250 μg/ml concentration range. 2.186 & 6.626 0.25-1.53 0.86-1.68 inter-day respectively.
 Conclusion: Here we conclude that methods are accurate, sensitive, reproducible quantitative its formulation. can used industries routine Favipiravir, particular RP-HPLC. main features proposed economic eco-friendly less retention time around 5.0 min.